罗尔斯通氏菌株及其在石油污染盐碱土壤生物修复中的应用

Ralstoniasp. and application thereof in bioremediation of petroleum-contaminated saline-alkali soil

Abstract

The invention discloses a Ralstoniasp. and application thereof in bioremediation of petroleum-contaminated saline-alkali soil. The Ralstoniasp. is collected by China General Microbiological Culture Collection Center (CGMCC), the preservation data is 4 November, 2009, and the preservation number is CGMCCNo.3389. The Ralstoniasp. is grown on an LB solid culture medium to form a white bacterial colony which is round and has regular edge and dry surface and is non-transparent; and the bacterial colony is in a round bar shap, dose not generate spores, is Gram-negative and has the highest growth temperature of 50 DEG C and the NaCl tolerance of 0-5%. The invention also discloses a nucleotide sequence of the Ralstoniasp. The Ralstoniasp. of the invention can degrade pollutants, such as petroleumhydrocarbon, polyaromatic hydrocarbon and the like in soil under the high salt environment; and the crude oil degradation rate can be above 60.0%. Thus, the invention can be applied in the biological control technology of soil pollution brought by polyaromatic hydrocarbon and petroleum.
本发明公开了一种罗尔斯通氏菌株(Ralstonia sp.)及其在石油污染盐碱土壤生物修复中的应用。该菌株的保藏单位:中国微生物菌种保藏管理委员会普通微生物中心,保藏日:2009年11月04日,保藏号CGMCC No.3389。该菌株在LB固体培养基上生长,形成的菌落特征为菌落呈白色,菌落为圆形、边缘规则、表面干燥、不透明,在显微镜下观察到菌落呈圆杆状,不产芽孢,革兰氏染色阴性,生长温度最高为50℃,NaCl耐受性0-5%。本发明还公开了该菌株的核苷酸序列,本发明的菌株可以在高盐环境中降解土壤中的石油烃和多环芳烃等污染物,对原油的降解率可达60.0%以上,可以应用在由多环芳烃和石油带来的土壤污染生物治理技术中。

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Patent Citations (1)

    Publication numberPublication dateAssigneeTitle
    CN-101717725-AJune 02, 2010中国海洋石油总公司;中国海洋大学;中海油能源发展股份有限公司;中海石油环保服务有限公司溢油污染海岸线生物修复用菌剂及制备方法

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